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F-SD-SCG-1002

Prenatal Screening Requisition

This requisition goes out at the first prenatal visit for almost every pregnant patient in Ontario. PHO screens for hepatitis B surface antigen, rubella immunity, syphilis and HIV from one serum tube, and it recommends offering HIV testing in every pregnancy. The results shape care for the rest of the pregnancy and for the newborn.

At a glance

Checked October 2026

Form number
F-SD-SCG-1002
Issued by
Public Health Ontario
Where
Ontario
Completed by
Ordering clinician
Format
Fillable PDF
Language
English

What the Prenatal Screening Requisition is for

The form orders Ontario’s routine prenatal serology at Public Health Ontario’s laboratory: hepatitis B surface antigen, rubella IgG, syphilis and, as an option, HIV. PHO says all pregnant patients should be screened for these four, and recommends offering HIV testing in each pregnancy regardless of apparent risk, since infection can happen between pregnancies.

Syphilis screening is recommended in the first trimester or at the first prenatal visit, and PHO suggests considering repeat screening later in pregnancy for patients at ongoing or high risk. Hepatitis A testing isn’t part of the screen and won’t be run unless clinically indicated.

Who completes it

The ordering clinician completes the whole form, giving their initial, surname and OHIP or CPSO number in the submitter section. A second doctor or clinic can be copied on results. The form’s own header says all sections must be completed at every visit, so use a fresh requisition each time you order.

How to fill in the requisition

1. Submitter and cc Doctor Information

Give your name, address, telephone, fax and courier code if you have one. Add the copy-to doctor’s name, clinic, CPSO number and address when someone else, such as the obstetrician or midwife, needs the results.

2. Patient Information

Use the names as they appear on the health card, with the health card number, date of birth, address and phone. Record the date collected and your submitter lab number. The tube must carry the patient’s first and last name, the collection date and one other identifier, and they must match the form or the specimen is rejected.

3. Test(s) Requested

Tick hepatitis B surface antigen, rubella, syphilis and HIV as needed. If rubella was done at another laboratory, tick that box so it isn’t repeated. If you’re asking for hepatitis B immunity for a specific exposure, write the reason on the form.

Common reasons it’s rejected or delayed

  • Hepatitis B immunity requested with no stated reason.
  • Labels on the tube that don’t match the requisition.
  • Specimens from donor screening, which PHO doesn’t offer.
  • Haemolysed, icteric, lipemic or contaminated serum.
  • Glass tubes, which PHO asks you not to send.
  • Other tests added to the form instead of their own requisition and specimen.

Submitting and who pays

If the blood is drawn in your office, send the tube and requisition to your local Public Health Ontario laboratory. If the patient goes to a lab for collection, send the completed requisition with them. Turnaround is up to three days from receipt, and up to six for a reactive hepatitis B, syphilis or HIV screen. PHO only performs insured services under Ontario’s Health Insurance Act, so the patient isn’t charged.

Get the official form

Download the current version from Public Health Ontario each time. Forms change without notice, and an old edition can be returned.

Official page ↗ PDF ↗

Medulla Forms

Fill F-SD-SCG-1002 from the visit and the chart.

Upload the fillable PDF to your practice's forms once. Medulla fills it from the visit and the chart open in your EMR, leaves blank anything the record doesn't support, and opens the form for your review. When it's done, save it to the patient's chart in one click.

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Sources for this guide

Questions

Questions we hear a lot.

Can I add hepatitis B immunity (anti-HBs) to the prenatal screen?

Not as routine. PHO rejects hepatitis B immunity testing on prenatal screens unless the requisition gives a clear reason, such as exposure to a case or a needlestick injury. HBsAg is the screening test for active infection.

Does the patient need a separate tube for each test?

No. One full red top or serum separator tube is enough for all four tests. If a full tube can't be drawn, PHO asks for two.

Can I order hepatitis C or other tests on this form?

No. For any other test, use the matching PHO requisition and send a separate specimen. HIV can also be ordered on its own with the HIV Serology and PCR requisition.

Can I get urgent results for a patient in labour?

PHO offers STAT testing only for HBsAg and HIV serology, and only for individuals in labour with no prenatal testing who are at high risk for hepatitis B or HIV.

Is patient information secure?

Yes. Medulla has a SOC 2 Type II report from an independent auditor, is HIPAA, PIPEDA, and PHIPA compliant, and is built and hosted in Canada, so patient data stays in the country and never gets pasted into consumer AI tools. Everything is encrypted in transit and at rest. See how we protect patient data.

More questions? Browse help.medulla.ca.