What the flow cytometry requisition is for
It names the flow cytometry test you want and why. The menu covers HLA-B27, CD4/CD8, B lymphocytes (CD19), NK lymphocytes (CD16/CD56), CD57, a lymphoproliferative disorder panel, an acute leukemia panel, and PNH. It attaches to the Ministry of Health lab requisition and doesn’t replace it.
Who completes it
The ordering healthcare provider completes the form. Collection staff record the date and time of service.
How to fill in the requisition
Healthcare provider and patient information
Give your full name, address, and a contact number the lab can reach for a critical result or a question, plus your CPSO or billing number. Patient details include date of birth, sex, and the health card number and version.
Test and clinical indication
Tick the test and write the clinical indication beside it. For the lymphoproliferative panel, the form lists unexplained lymphocytosis, suspected lymphoma, CLL, Sézary syndrome and T-LGL as indications. The acute leukemia panel is for circulating blasts, unexplained cytopenias, or transformation of MDS or MPD. A CBC finding or the reason for the HLA-B27 helps the lab choose the right markers.
Sample requirements
Blood and marrow go in a 4 mL EDTA tube. Lymph node or other tissue goes in a sterile container covered with normal saline, and fine needle aspirates in a sterile container. Ship everything at room temperature.
Common reasons it’s delayed
- Collected on a Friday or weekend.
- Sent without the Ministry of Health requisition.
- No clinical indication for a lymphoproliferative or leukemia panel.
- Tissue sent in formalin instead of saline.
- No reachable contact number for a critical result.
Submitting and who pays
The patient attends a Dynacare collection site with both requisitions, or the clinic sends the sample with them. The flow cytometry form has no payer section, so billing follows the Ministry of Health requisition it is attached to.